Abstract: The main objective of the study was the extraction and testing of the antimicrobial activity of the Lemon bark extract against the microorganisms involved in air and waterborne infections. The antibiotics are not very effective in the present day as the microorganisms are becoming resistant, the study concentrates on the antimicrobial activity and time ahead of the prophylactic potential of the lemon bark extract. Phytochemical analysis for the constituents of Lemon bark showed the presence of saponins, flavonoids and tannins in the aqueous form of solution. The potency test on microorganisms proved to be active against Salmonella shigella with the minimum inhibitory concentration of 100 mg/L was 16.27 mm, 150 mg/L was 16.43 mm and 250 mg/L was 16.73 mm. Meanwhile, for Escherichia coli for 100 mg/L was 13.20 mm, 100 mg/L was 13.10 mm and 150 mg/L was 13.13 mm. Show less...

Abstract: Herbal teas are very common around the world. It has a good therapeutic effect. But due to adulteration, we face an obstacle to using them. The author has collected some clinical trials on commercial herbal tea formulations on the market that have proven their efficacy and safety. So, the future is for herbal medicine owing to people's psychology, minimum adverse reactions, and lower cost. The pharmaceutical manufacturing of herbal tea formulations should be encouraged to use them as add-on therapy or healthy daily beverages at least. Show less...

Abstract: Any product generated from food sources that offer additional health advantages over and above the essential nutritional content present in foods is referred to as a nutraceutical under the broad umbrella term. The catechins in Camellia sinensis (Theaceae) namely (-)-epicatechin, (-)-epicatechin-3-gallate, (-)-epigallocatechin, and (-)-epigallocatechin-3-gallate (EGCG), which can be used as nutraceuticals in food or as a component of food items has been discussed. Catechins being polyphenols and antioxidants are found to have a wide range of therapeutic application like weight loss, anticancer, anti-inflammatory, and a few more therapeutic applications, through various mechanisms like stimulating AMP-activated protein kinase, enhanced apoptosis, decreased expression of interleukin (IL)-6 and IL-8. The most recent method for extracting catechins include combining the extraction processing of PEF or IPL with Subcritical water extraction. Food additives have been mixed with green tea extracts to develop a desired formulation like chewing gum and capsule. As green tea catechins are a beneficial phytoconstituents to improve overall health, its prospects include formulation of a gelatin gummy formulation which will improve its palatability by masking the bitter taste. Gelatin gummy formulation can be carried conveniently and will provide easy access to the consumer as compared to green tea. Furthermore, we found a scope to develop an analytical method for EGCG and carry out its validation by HPLC which will be more reliable and cost-efficient in comparison to the existing UHPLC methods for EGCG. Show less...

Abstract: The well-known tropical tree Psidium guajava, also known as the guava, is widely farmed for its fruit. This article consists of literature about various analytical techniques like TLC, UV spectrophotometry, HPLC, and HPTLC that could be used to analyze the Guava plant. The HPLC analysis of Quercetin isolated from the leaves of the guava plant uses a 4 x 125 mm Hypersil ODS column with a mobile phase of 0.5 percent ortho-phosphoric acid in water and methanol at a flow rate of 1ml/min. The column wash times are 20 min and 18 min, respectively. 250C is the temperature in the column. Silica gel 60F 254 pre-coated plates (10x10 cm) were used with toluene: acetone: formic acid (38:10:5) as the solvent system for HPTLC analysis of quercetin obtained from the tender leaves of guava. The λ max was observed in the U.V. spectrum of the isolated compound (quercetin) on three standard wavelengths: 212 nm, 256 nm, and 372 nm. An effort can be made to produce a new analytical method that will be cost-effective and time-saving. Show less...